Anti-P27 KIP1 antibody

Product Name Anti-P27 KIP1 antibody

Catalog No PAab06068

Packing 100ug

Form liquid

Alternative Name CDKN1B antibody, CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27 antibody, P27 antibody, KIP1 antibody, P27KIP1

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Immunogen cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Specificity Human, Mouse

Tested Application ELISA,WB,IP,IHC,IF

Recommended dilution WB : 1:200-1:2000 IP : 1:200-1:1000 IHC : 1:50-1:500 IF : 1:50-1:500

IHC use

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using P27; KIP1 Antibody at dilution of 1:200 under 10x lens). heat mediated antigen retrieved with Tris-EDTA bufferpH9).

WB use

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with PAab06068( P27; KIP1 Antibody) at dilution of 1:600

Protein Information

Gene ID1027

Uniprot ID P46527

Calculated MW 27 kDa

location