Anti-PARN antibody

Product Name Anti-PARN antibody

Catalog No PAab06150

Packing 100ug

Form liquid

Alternative Name DAN

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.

Immunogen poly(A)-specific ribonuclease (deadenylation nuclease)

Specificity Human, Mouse ,Rat

Tested Application ELISA,WB,IHC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human endometrial cancer usingPARN antibody at dilution of 1:100

WB use

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with PAab06150(PARN antibody) at dilution of 1:500

Protein Information

Gene ID5073

Uniprot ID O95453

Calculated MW

location