Anti-PARN antibody
Product Name Anti-PARN antibody
Catalog No PAab06150
Packing 100ug
Form liquid
Alternative Name DAN
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
Immunogen poly(A)-specific ribonuclease (deadenylation nuclease)
Specificity Human, Mouse ,Rat
Tested Application ELISA,WB,IHC
Recommended dilution
IHC use
Immunohistochemistry of paraffin-embedded human endometrial cancer usingPARN antibody at dilution of 1:100
WB use
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with PAab06150(PARN antibody) at dilution of 1:500
Protein Information
Gene ID5073
Uniprot ID O95453
Calculated MW
location