Anti-ROCK2 antibody

Product Name Anti-ROCK2 antibody

Catalog No PAab07378

Packing 100ug

Form liquid

Alternative Name KIAA0619

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Immunogen Rho-associated, coiled-coil containing protein kinase 2

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC,IF,IP

Recommended dilution WB:1:1000-1:10000 IP:1:200-1:2000 IHC:1:20-1:200 IF:1:10-1:100

IHC use

Immunohistochemistry of paraffin-embedded human liver usingROCK2 antibody at dilution of 1:100

WB use

HT-1080 cells were subjected to SDS PAGE followed by western blot with PAab07378(ROCK2 antibody) at dilution of 1:1000

Protein Information

Gene ID9475

Uniprot ID O75116

Calculated MW 161kDa

location