Product Name Anti-RUVBL1 antibody
Catalog No PAab07537
Alternative Name INO80H antibody, NMP238 antibody, TIP49 antibody, TIP49A
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation. May be able to bind plasminogen at cell surface and enhance plasminogen activation.
Immunogen RuvB-like 1 (E. coli)
Specificity Human,Mouse ,Rat
Tested Application ELISA,WB,IHC,IP
Immunohistochemistry of paraffin-embedded mouse liver tissue slide using RUVBL1 Antibody at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA bufferpH9).
HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab07537(RUVBL1 antibody) at dilution of 1:1000
Uniprot ID Q9Y265