Anti-Vimentin antibody

Product Name Anti-Vimentin antibody

Catalog No PAab09408

Packing 100ug

Form liquid

Alternative Name FLJ36605 antibody, VIM antibody, Vimentin

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Immunogen vimentin

Specificity Human,Mouse ,Rat

Tested Application ELISA,IF,IHC,WB,FC

Recommended dilution WB :1:1000-1:10000;IHC:1:20-1:500;IF:1:50-1:500

IHC use

Immunohistochemistry of paraffin-embedded human tonsillitis usingVIM antibody at dilution of 1:50

WB use

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with PAab09408(VIM antibody) at dilution of 1:2000

Protein Information

Gene ID7431

Uniprot ID P08670

Calculated MW 55kDa

location