Rabbit Anti- Human acetylserotonin O-methyltransferase, ASMT polyclonal Antibody

FOR RESEARCH USE ONLY.

PROUDUCT INFORMATION

Catalog Number: PA-RA-07956

Size: 0.2ml (200ug/ml)

Immunogen: Synthetic peptide-KLH

Ig type: rabbit IgG

BACKGROUND  

Melatonin is a natually occurring secreted hormone that exhibits antioxidant capabilities and is crucial in the proper regulation of the circadian rhythm.ASMT (acetylserotonin O-methyltransferase), also known as HIOMT (hydroxyindole O-methyltransferase), is a 345 amino acid protein that belongs to the methyltransferase superfamily and is involved in melatonin biosynthesis.Expressed in brain, retina and pineal gland, ASMT functions to catalyze the final reaction in the synthesis of melatonin, specifically the conversion of S-adenosyl-L-methionine and N-acetylserotonin to S-adenosyl-L-homocysteine and melatonin. Multiple isoforms of ASMT exist due to alternative splicing events. The gene encoding ASMT maps to the pseudoautosomal region of chromosomes X and Y..

SPEFICITY    

Anti-ASMT antibody is a rabbit polyclonal antibody raised against ASMT. This antibody has been selected for its ability to recognize ASMT in immunohistochemistry staining and ELISA.

APPLICATION   

Immunocytochemistry in formalin fixed cells:  1:50-200

Immunohistochemistry in formalin fixed frozen section:  1:50-200

Immunohistochemistry  in paraffin section:  1:50-200

Optimal working dilutions must be determined by end user.                                                                                                                                                             

CONTENTS     

0.01M PBS, 30%glycerol.

STORAGE      

Store at 4° C for frequent use. Reconstituted antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

REFERENCE     

1.        Toma, C., et al. 2007. Is ASMT a susceptibility gene for autism spectrum disorders? A replication study in European populations. Mol. Psychiatry12: 977-979.

2.      Rodriguez, I.R., et al. 1994. Structural analysis of the human hydroxyindole-O-methyltransferase gene. Presence of two distinct promoters. J.Biol. Chem. 269: 31969-31977