Porcine Alpha lactalbumin, LALBA ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Alpha-lactalbumin,Lactose synthase B protein, LALBA,lactose synthase B protein; alpha-lactalbumin; lysozyme-like protein 7; lactalbumin alpha

Search name

Porcine Alpha-lactalbumin ELISA KIT ,Porcine Lactose synthase B protein ELISA KIT ,Porcine LALBA ELISA KIT ,Porcine lactose synthase B protein ELISA KIT ,Porcine alpha-lactalbumin ELISA KIT ,Porcine lysozyme-like protein 7 ELISA KIT ,Porcine lactalbumin alpha ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine alpha-lactalbumin,Alpha-lactalbumin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-lactalbumin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-lactalbumin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-lactalbumin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-lactalbumin in the samples is then determined by comparing the O.D. of the samples to the standard curve.