Porcine Apolipoprotein A-I, APOA1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

APOA1,apoA-I,apo-AI; apolipoprotein A-I,Apolipoprotein A1,

Search name

Porcine APOA1 ELISA KIT ,Porcine apoA-I ELISA KIT ,Porcine apo-AI ELISA KIT ,Porcine apolipoprotein A-I ELISA KIT ,Porcine Apolipoprotein A1 ELISA KIT 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of porcine Apo-AI concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo-AI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo-AI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo-AI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apo-AI in the samples is then determined by comparing the O.D. of the samples to the standard curve.