Porcine Arginase- 1, ARG1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ARG1,arginase liver; liver-type arginase; type I arginase; arginase 1, Arginase-1,

Search name

Porcine ARG1 ELISA KIT ,Porcine arginase liver ELISA KIT ,Porcine liver-type arginase ELISA KIT ,Porcine type I arginase ELISA KIT ,Porcine arginase 1 ELISA KIT ,Porcine Arginase-1 ELISA KIT  

Intended use

This immunoassay kit allows for the in vitro quantitative determination of porcine Arginase-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginase-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for Arginase-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Arginase-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Arginase-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.