Porcine Calpain-1 catalytic subunit, CAPN1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CAPN1,CANP; CANP1; CANPL1; muCANP; muCL; CANP 1; calcium-activated neutral proteinase 1; calpain mu-type; calpain, large polypeptide L1; calpain-1 catalytic subunit; calpain-1 large subunit; cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; micromolar-calpain; calpain 1 (mu/I) large subunit

Search name

Porcine CAPN1 ELISA KIT ,Porcine CANP ELISA KIT ,Porcine CANP1 ELISA KIT ,Porcine CANPL1 ELISA KIT ,Porcine muCANP ELISA KIT ,Porcine muCL ELISA KIT ,Porcine CANP 1 ELISA KIT ,Porcine calcium-activated neutral proteinase 1 ELISA KIT ,Porcine calpain mu-type ELISA KIT ,Porcine calpain ELISA KIT ,Porcine  large polypeptide L1 ELISA KIT ,Porcine calpain-1 catalytic subunit ELISA KIT ,Porcine calpain-1 large subunit ELISA KIT ,Porcine cell proliferation-inducing gene 30 protein ELISA KIT ,Porcine cell proliferation-inducing protein 30 ELISA KIT ,Porcine micromolar-calpain ELISA KIT ,Porcine calpain 1 (mu/I) large subunit ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Calpain-1 catalytic subunit concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain-1 catalytic subunit. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Calpain-1 catalytic subunit and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Calpain-1 catalytic subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Calpain-1 catalytic subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.