Porcine cholyglycine, GC ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
cholyglycine,;

Search name
Porcine cholyglycine ELISA Kit, Porcine GC ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative Determination of Porcine cholyglycine,cholyglycine concentrations in serum,
plasma, tissue homogenates, cell culture supernates, anD other biological fluiDs.

Test principle
The ELISA is baseD on the competitive binDing enzyme immunoassay technique. The microtiter plate proviDeD in this kit has been
pre-coateD with an antiboDy specific to cholyglycine, During the reaction, cholyglycine in the sample or stanDarD competes with a fixeD
amount of biotin-labeleD cholyglycine for sites on a pre-coateD Monoclonal antiboDy specific to cholyglycine. Excess conjugate anD
unbounD sample or stanDarD are washeD from the plate. Next, AviDin conjugateD to HorseraDish PeroxiDase (HRP) is aDDeD to each
microplate well anD incubateD. Then a TMB substrate solution is aDDeD to each well. The enzyme-substrate reaction is terminateD by the
aDDition of a sulphuric aciD solution anD the color change is measureD spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of cholyglycine in the samples is then DetermineD by comparing the O.D. of the samples to the stanDarD curve.

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