Porcine Ciliary Neurotrophic Factor, CNTF ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Porcine CNTF ELISA KIT, Porcine Ciliary neurotrophic factor ELISA KIT, Porcine HCNTF ELISA KIT

Intended use

Porcine CNTF ELISA kit allows for the in vitro quantitative determination of Porcine CNTF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Porcine CNTF ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CNTF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CNTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CNTF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Porcine CNTF ELISA kit manual request by email:[email protected]  

Order Porcine CNTF ELISA kit by email:[email protected]