Porcine Deoxyribonuclease- 1, DNASE1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Deoxyribonuclease-1,Deoxyribonuclease I,DNase I, DNASE1,deoxyribonuclease I,DNL1; DRNI; DNase I, lysosomal; Dornase alfa; deoxyribonuclease-1; Porcine urine deoxyribonuclease I

Search name

Porcine Deoxyribonuclease-1 ELISA KIT ,Porcine Deoxyribonuclease I ELISA KIT ,Porcine DNase I ELISA KIT ,Porcine DNASE1 ELISA KIT ,Porcine deoxyribonuclease I ELISA KIT ,Porcine DNL1 ELISA KIT ,Porcine DRNI ELISA KIT ,Porcine DNase I lysosomal ELISA KIT ,Porcine Dornase alfa ELISA KIT ,Porcine deoxyribonuclease-1 ELISA KIT ,Porcine Porcine urine deoxyribonuclease I ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Deoxyribonuclease-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deoxyribonuclease-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Deoxyribonuclease-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Deoxyribonuclease-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Deoxyribonuclease-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.