Porcine Dystrophin, DMD ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Dmd,Dystrophin,BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85
Search name
Porcine Dmd ELISA KIT ,Porcine Dystrophin ELISA KIT ,Porcine BMD ELISA KIT ,Porcine CMD3B ELISA KIT ,Porcine DXS142 ELISA KIT ,Porcine DXS164 ELISA KIT ,Porcine DXS206 ELISA KIT ,Porcine DXS230 ELISA KIT ,Porcine DXS239 ELISA KIT ,Porcine DXS268 ELISA KIT ,Porcine DXS269 ELISA KIT ,Porcine DXS270 ELISA KIT ,Porcine DXS272 ELISA KIT ,Porcine MRX85 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of porcine Dystrophin concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrophin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dystrophin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dystrophin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dystrophin in the samples is then determined by comparing the O.D. of the samples to the standard curve.