Porcine Endothelial NOS, eNOS ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Nitric oxide synthase, endothelial,NOS type III(NOSIII),Endothelial NOS(eNOS),Constitutive NOS(cNOS),EC-NOS, nitric oxide synthase,
endothelial; nitric oxide synthase 3 (endothelial cell)

Search name
Porcine Endothelial nitric oxide synthase 3 ELISA Kit, Porcine eNOS-3 ELISA Kit, Porcine Nitric oxide synthase endothelial ELISA
Kit, Porcine NOS type III ELISA Kit, Porcine NOSIII ELISA Kit, Porcine Endothelial NOS ELISA Kit, Porcine eNOS ELISA Kit, Porcine
Constitutive NOS ELISA Kit, Porcine cNOS ELISA Kit, Porcine EC-NOS ELISA Kit, Porcine EC-NOS ELISA Kit, Porcine nitric oxide
synthase 3 ELISA Kit, Porcine endothelial cell ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine nitric oxide synthase, endothelial,Nitric oxide synthase,
endothelial concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric oxide synthase, endothelial. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Nitric
oxide synthase, endothelial and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Nitric oxide synthase, endothelial, biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Nitric oxide synthase, endothelial in the samples is then determined by comparing the O.D. of the samples to the standard curve.