Porcine Heat Shock Protein 70, HSP70 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Heat shock 70 kDa protein 1A/1B,;Heat shock 70 kDa protein 1/2(HSP70-1/HSP70-2,HSP70.1/HSP70.2), Hsp70,eat shock 70 kDa
protein 1A/1B; HSP70-1; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1B; HSP70-1/HSP70-2; HSP70.1/HSP70.2; dnaK-type molecular
chaperone HSP70-1; heat shock 70 kDa protein 1/2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70kDa protein
1A

Search name
Porcine Heat shock 70 kDa protein 1A ELISA Kit, Porcine Heat shock 70 kDa protein 1B ELISA Kit, Heat shock 70 kDa protein 1A
ELISA Kit, Heat shock 70 kDa protein 1B ELISA Kit, Porcine Heat Shock Protein 70 ELISA Kit, Porcine HSP- 70 ELISA Kit , Heat
Shock Protein 70 ELISA Kit, HSP- 70 ELISA Kit ,HSPA1 ELISA Kit ,HSPA1B ELISA Kit ,HSPA1A ELISA Kit ,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine heat shock 70 kda protein 1a,Heat shock 70 kDa protein
1A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat shock 70 kDa protein 1A. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Heat
shock 70 kDa protein 1A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a
TMB substrate solution is added to each well. Only those wells that contain Heat shock 70 kDa protein 1A, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Heat shock 70
kDa protein 1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.