Porcine hemoglobin A1c Glycated, HbA1c ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Glycosylated hemoglobin A1c, hemoglobin A1c, HbA1c, A1C, Hb1c; HbA1c

Search name

Porcine Glycosylated hemoglobin A1c ELISA KIT ,Porcine hemoglobin A1c ELISA KIT ,Porcine HbA1c ELISA KIT ,Porcine A1C ELISA KIT ,Porcine Hb1c ELISA KIT ,Porcine HbA1c ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine glycosylated hemoglobin a1c,Glycosylated hemoglobin A1c concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycosylated hemoglobin A1c. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Glycosylated hemoglobin A1c and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycosylated hemoglobin A1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glycosylated hemoglobin A1c in the samples is then determined by comparing the O.D. of the samples to the standard curve.