Porcine Interleukin- 4, IL- 4 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Interleukin-4(IL-4),B-cell stimulatory factor 1(BSF-1),Lymphocyte stimulatory factor 1, BCGF-1; BCGF1; BSF-1; BSF1; B cell growth
factor 1; B cell stimulatory factor 1; binetrakin; interleukin-4; lymphocyte stimulatory factor 1; pitrakinra; Interleukin 4

Search name
Porcine Interleukin-4 ELISA Kit, Porcine IL-4 ELISA Kit, Porcine B-cell stimulatory factor 1ELISA Kit, Porcine BSF-1 ELISA Kit,
Porcine Lymphocyte stimulatory factor 1 ELISA Kit, Porcine BCGF-1 ELISA Kit, Porcine BCGF1 ELISA Kit, Porcine BSF1 ELISA
Kit, Porcine B cell stimulatory factor 1 ELISA Kit, Porcine binetrakin ELISA Kit, Porcine pitrakinra ELISA Kit, Porcine Interleukin
4 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine interleukin-4,IL-4 concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-4. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-4 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain IL-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-4 in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

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