Porcine Leukocyte elastase inhibitor, SERPINB1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Leukocyte elastase inhibitor, Serpin B1,Monocyte/neutrophil elastase inhibitor,Peptidase inhibitor 2,SERPINB1,ELANH2;EI,HEL57; LEI; M/NEI; MNEI; PI-2; PI2; epididymis luminal protein 57; leukocyte elastase inhibitor; peptidase inhibitor 2; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine proteinase inhibitor clade B member 1; serpin peptidase inhibitor clade B member 1

Search name

Porcine Leukocyte elastase inhibitor ELISA KIT ,Porcine  Serpin B1 ELISA KIT ,Porcine Monocyte/neutrophil elastase inhibitor ELISA KIT ,Porcine Peptidase inhibitor 2 ELISA KIT ,Porcine SERPINB1 ELISA KIT ,Porcine ELANH2 ELISA KIT ,Porcine EI ELISA KIT ,Porcine HEL57 ELISA KIT ,Porcine LEI ELISA KIT ,Porcine M/NEI ELISA KIT ,Porcine MNEI ELISA KIT ,Porcine PI-2 ELISA KIT ,Porcine PI2 ELISA KIT ,Porcine epididymis luminal protein 57 ELISA KIT ,Porcine leukocyte elastase inhibitor ELISA KIT ,Porcine peptidase inhibitor 2 ELISA KIT ,Porcine protease inhibitor 2 (anti-elastase) ELISA KIT ,Porcine  monocyte/neutrophil derived ELISA KIT ,Porcine serine proteinase inhibitor clade B member 1 ELISA KIT ,Porcine serpin peptidase inhibitor clade B member 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine leukocyte elastase inhibitor,LEI concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LEI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LEI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LEI in the samples is then determined by comparing the O.D. of the samples to the standard curve.