Porcine luteotropic hormone, LH ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Lutropin subunit beta(Lutropin beta chain),Luteinizing hormone subunit beta(LH-B,LSH-B,LSH-beta), LH,CGB4; LSH-B; hLHB; LSH-beta;
interstitial cell stimulating hormone, beta chain; luteinizing hormone beta subunit; lutropin beta chain; lutropin subunit beta; luteinizing
hormone beta polypeptide

Search name
Poricne Lutropin subunit beta ELISA Kit, Poricne LHB ELISA Kit, Poricne Lutropin beta chain ELISA Kit, Poricne Luteinizing
hormone subunit beta ELISA Kit, Poricne LH-B ELISA Kit, Poricne LSH-B ELISA Kit, Poricne LSH-beta ELISA Kit, Poricne LH
ELISA Kit, Poricne CGB4ELISA Kit, Poricne hLHB ELISA Kit, Poricne interstitial cell stimulating hormone, beta chain ELISA Kit,
Poricne luteinizing hormone beta polypeptide ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Poricne lutropin subunit beta,LHB concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to LHB. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LHB and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain LHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LHB in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

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