Porcine Mitochondrial uncoupling protein 2,UCP 2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
UCP2,uncoupling protein 2,BMIQ4; SLC25A8; UCPH; solute carrier family 25 member 8; uncoupling protein 2
Search name
Porcine UCP2 ELISA KIT ,Porcine uncoupling protein 2 ELISA KIT ,Porcine BMIQ4 ELISA KIT ,Porcine SLC25A8 ELISA KIT ,Porcine UCPH ELISA KIT ,Porcine solute carrier family 25 member 8 ELISA KIT ,Porcine uncoupling protein 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of porcine mitochondrial uncoupling protein 2,UCP 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to UCP 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for UCP 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UCP 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UCP 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.