Porcine Major histocompatibility complex class I- related gene protein, MR1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MR1,major histocompatibility complex class I-related gene protein; HLALS; MHC class I-like antigen MR-1; MHC class I-related gene protein; MHC class-I related-gene protein; class I histocompatibility antigen-like protein; major histocompatibility complex, class I-like sequence; major histocompatibility complex, class I-related

Search name

Porcine MR1 ELISA KIT ,Porcine major histocompatibility complex class I-related gene protein ELISA KIT ,Porcine HLALS ELISA KIT ,Porcine MHC class I-like antigen MR-1 ELISA KIT ,Porcine MHC class I-related gene protein ELISA KIT ,Porcine MHC class-I related-gene protein ELISA KIT ,Porcine class I histocompatibility antigen-like protein ELISA KIT ,Porcine major histocompatibility complex ELISA KIT ,Porcine  class I-like sequence ELISA KIT ,Porcine major histocompatibility complex ELISA KIT ,Porcine class I-related ELISA KIT

Intended use

Porcine MR1 ELISA KIT allows for the in vitro quantitative determination of porcine major histocompatibility complex class i-related gene protein,MHC class I-related gene protein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Porcine MR1 ELISA KIT has been pre-coated with an antibody specific to MHC class I-related gene protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MHC class I-related gene protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MHC class I-related gene protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MHC class I-related gene protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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