Porcine Myoblast determination protein 1, MYOD1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MYOD1,MyoD,MYF3; PUM; bHLHc1; class C basic helix-loop-helix protein 1; myf-3; myoblast determination protein 1; myogenic factor 3; myogenic differentiation 1,Class C basic helix-loop-helix protein 1(bHLHc1);Myogenic factor 3(Myf-3)

Search name

Porcine MYOD1 ELISA KIT ,Porcine MyoD ELISA KIT ,Porcine MYF3 ELISA KIT ,Porcine PUM ELISA KIT ,Porcine bHLHc1 ELISA KIT ,Porcine class C basic helix-loop-helix protein 1 ELISA KIT ,Porcine myf-3 ELISA KIT ,Porcine myoblast determination protein 1 ELISA KIT ,Porcine myogenic factor 3 ELISA KIT ,Porcine myogenic differentiation 1 ELISA KIT ,Porcine Class C basic helix-loop-helix protein 1 ELISA KIT ,Porcine bHLHc1;Myogenic factor 3 ELISA KIT ,Porcine Myf-3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Myoblast determination protein 1, MYOD1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYOD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MYOD1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MYOD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MYOD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.