Porcine Propionyl CoA carboxylase beta chain mitochondrial, PCCB ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Class 6.4.1.3, PCCase subunit beta,Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCB,propionyl-CoA carboxylase beta chain mitochondrial; PCCase subunit beta; propanoyl-CoA: propionyl Coenzyme A carboxylase beta polypeptide; propionyl CoA carboxylase beta polypeptide

Search name

Porcine Class 6.4.1.3 ELISA KIT ,Porcine PCCase subunit beta ELISA KIT ,Porcine Propanoyl-CoA:carbon dioxide ligase subunit beta ELISA KIT ,Porcine PCCB ELISA KIT ,Porcine propionyl-CoA carboxylase beta chain mitochondrial ELISA KIT ,Porcine PCCase subunit beta ELISA KIT ,Porcine propanoyl-CoA: propionyl Coenzyme A carboxylase beta polypeptide ELISA KIT ,Porcine propionyl CoA carboxylase beta polypeptide ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine propionyl-coa carboxylase beta chain, mitochondrial,PCCase subunit beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCCase subunit beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PCCase subunit beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PCCase subunit beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PCCase subunit beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.