Porcine Plasminogen activator inhibitor 1,PAI ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Plasminogen activator inhibitor 1,PAI,PAI-1, Mr1,Pai1,Planh1,Endothelial plasminogen activator inhibitor,Serpin E1, Serpine1,PAI-1,PAI; PAI1; PLANH1; endothelial plasminogen activator inhibitor; plasminogen activator inhibitor 1; serineproteinase inhibitor clade E member 1; serpin E1; serpin peptidase inhibitor nexin plasminogen activator inhibitor type 1 member 1

Search name

Porcine Plasminogen activator inhibitor 1 ELISA KIT ,Porcine PAI ELISA KIT ,Porcine PAI-1 ELISA KIT ,Porcine  Mr1 ELISA KIT ,Porcine Pai1 ELISA KIT ,Porcine Planh1 ELISA KIT ,Porcine Endothelial plasminogen activator inhibitor ELISA KIT ,Porcine Serpin E1 ELISA KIT ,Porcine Serpine1 ELISA KIT ,Porcine PAI-1 ELISA KIT ,Porcine PAI ELISA KIT ,Porcine PAI1 ELISA KIT ,Porcine PLANH1 ELISA KIT ,Porcine endothelial plasminogen activator inhibitor ELISA KIT ,Porcine plasminogen activator inhibitor 1 ELISA KIT ,Porcine serineproteinase inhibitor clade E member 1 ELISA KIT ,Porcine serpin E1 ELISA KIT ,Porcine serpin peptidase inhibitor nexin plasminogen activator inhibitor type 1 member 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine plasminogen activator inhibitor 1,PAI concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PAI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PAI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PAI in the samples is then determined by comparing the O.D. of the samples to the standard curve.