Porcine Prelamin-A/C,LMNA ELISA KIT

Porcine Prelamin- A/C, LMNA ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

LMNA,lamin A/C,CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; PRO1; 70 kDa lamin; lamin A/C-like 1; prelamin-A/C; renal carcinoma antigen NY-REN-32

Search name

Porcine LMNA ELISA KIT ,Porcine lamin A/C ELISA KIT ,Porcine CDCD1 ELISA KIT ,Porcine CDDC ELISA KIT ,Porcine CMD1A ELISA KIT ,Porcine CMT2B1 ELISA KIT ,Porcine EMD2 ELISA KIT ,Porcine FPL ELISA KIT ,Porcine FPLD ELISA KIT ,Porcine FPLD2 ELISA KIT ,Porcine HGPS ELISA KIT ,Porcine IDC ELISA KIT ,Porcine LDP1 ELISA KIT ,Porcine LFP ELISA KIT ,Porcine LGMD1B ELISA KIT ,Porcine LMN1 ELISA KIT ,Porcine LMNC ELISA KIT ,Porcine LMNL1 ELISA KIT ,Porcine PRO1 ELISA KIT ,Porcine 70 kDa lamin ELISA KIT ,Porcine lamin A/C-like 1 ELISA KIT ,Porcine prelamin-A/C ELISA KIT ,Porcine renal carcinoma antigen NY-REN-32 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of PorcinePrelamin-A/Cconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prelamin-A/C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forPrelamin-A/C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Prelamin-A/C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Prelamin-A/C in the samples is then determined by comparing the O.D. of the samples to the standard curve.