Porcine Retinol- binding protein 3, RBP3 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
RBP3,D10S64; D10S65; D10S66; IRBP; RBPI; RP66; interphotoreceptor retinoid-binding protein; retinol-binding protein 3; retinol binding protein 3 interstitial
Search name
Porcine RBP3 ELISA KIT ,Porcine D10S64 ELISA KIT ,Porcine D10S65 ELISA KIT ,Porcine D10S66 ELISA KIT ,Porcine IRBP ELISA KIT ,Porcine RBPI ELISA KIT ,Porcine RP66 ELISA KIT ,Porcine interphotoreceptor retinoid-binding protein ELISA KIT ,Porcine retinol-binding protein 3 ELISA KIT ,Porcine retinol binding protein 3 interstitial ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine Retinol- binding protein 3, RBP3 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RBP3, During the reaction, RBP3 in the sample or standard competes with a fixed amount of biotin-labeled RBP3 for sites on a pre-coated Monoclonal antibody specific to RBP3. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RBP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.