Porcine SUCLG2 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name SUCLG2
Protein Name Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
Alternative Name
Porcine SUCLG2 ELISA KIT, Porcine G-SCS ELISA KIT ,Porcine GBETA ELISA KIT ,Porcine GTPSCS ELISA KIT ,Porcine succinate--CoA ligase [GDP-forming] subunit beta mitochondrial ELISA KIT ,Porcine GTP-specific succinyl-CoA synthetase beta subunit ELISA KIT ,Porcine GTP-specific succinyl-CoA synthetase subunit beta ELISA KIT ,Porcine SCS-betaG ELISA KIT ,Porcine succinyl-CoA ligase [GDP-forming] subunit beta mitochondrial ELISA KIT ,Porcine succinyl-CoA ligase GDP-forming beta chain mitochondrial ELISA KIT ,Porcine succinyl-CoA synthetase beta-G chain ELISA KIT ,Porcine succinate-CoA ligase GDP-forming beta subunit ELISA KIT
Intended use
Porcine SUCLG2 ELISA KIT allows for the in vitro quantitative determination of SUCLG2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20mL |
Assay Diluent A | 1 × 10mL |
Assay Diluent B | 1 × 10mL |
Detection Reagent A | 1 × 120μL |
Detection Reagent B | 1 × 120μL |
Wash Buffer(25 x concentrate) | 1 × 30mL |
Substrate | 1 × 10mL |
Stop Solution | 1 × 10mL |
Plate sealer | 5 |
Test principle
The microtiter plate provided in Porcine SUCLG2 ELISA KIT has been pre-coated with an SUCLG2 antibody specific to SUCLG2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SUCLG2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SUCLG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SUCLG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20