Porcine Superoxide dismutase, SOD ELISA Kit
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
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This immunoassay kit allows for the in vitro quantitative determination of Porcine superoxide dismutase [cu-zn],Superoxide dismutase
[Cu-Zn] concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide dismutase [Cu-Zn]. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Superoxide dismutase [Cu-Zn] and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Superoxide dismutase [Cu-Zn], biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Superoxide dismutase [Cu-Zn] in the samples is then determined by comparing the O.D. of the samples to the standard curve.