Porcine Transcobalamin- 1, TCN1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Transcobalamin-1,TC-1,Transcobalamin I,TC I,TCI,Transcobalamin-1,haptocorrin, vitamin B12 binding protein R binder family, TCO1

Search name

Porcine Transcobalamin-1 ELISA KIT ,Porcine TC-1 ELISA KIT ,Porcine Transcobalamin I ELISA KIT ,Porcine TC I ELISA KIT ,Porcine TCI ELISA KIT ,Porcine Transcobalamin-1 ELISA KIT ,Porcine haptocorrin ELISA KIT ,Porcine  vitamin B12 binding protein R binder family ELISA KIT ,Porcine TCO1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine transcobalamin-1,TC-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TC-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TC-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TC-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TC-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.