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pTT5 Plasmid

Model: PVT1032
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  • pTT5 Plasmid
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pTT5 Vector | High-Yield Mammalian Expression Plasmid | PVT1032

pTT5 Vector (pTT5 Plasmid)

Catalog No: PVT1032 | Availability: In Stock
Download Full Protocol (.PDF)

The pTT5 vector is an industry-standard mammalian expression plasmid optimized for HEK293-6E and CHO cells. Utilizing the oriP/EBNA1 system, it provides exceptional yields for transient protein and antibody expression, making it a cornerstone for modern biopharmaceutical research.

Technical Specifications

Vector Type Mammalian Expression / Transient
Promoter CMV (Cytomegalovirus)
Selection Marker Ampicillin (100 µg/ml)
Replicon pMB1, oriP
Full Size 4401 bp
Host Expression HEK293, CHO, HEK293-6E

Vector Map

pTT5 Plasmid Map

(Schematic Map of pTT5 Vector)

pTT5 Plasmid Sequence

Access the complete nucleotide sequence for pTT5 below (FASTA format):

Click to Expand Full Sequence 
>pTT5_Plasmid_Sequence_4401bp  LOCUS       Exported                4401 bp ds-DNA     circular SYN 08-JULY-2015  DEFINITION  synthetic circular DNA  KEYWORDS    pTT5  SOURCE      synthetic DNA construct    ORGANISM  synthetic DNA construct  REFERENCE   1  (bases 1 to 4401)    AUTHORS   Huanghua    TITLE     Direct Submission  FEATURES             Location/Qualifiers       source          1..4401                       /organism="synthetic DNA construct"                       /mol_type="other DNA"       enhancer        42..421                       /note="CMV enhancer"                       /note="human cytomegalovirus immediate early enhancer"       promoter        422..625                       /note="CMV promoter"                       /note="human cytomegalovirus (CMV) immediate early                       promoter"       polyA_site      1277..1332                       /note="beta-globin poly(A) signal"                       /note="rabbit beta-globin polyadenylation signal"       promoter        2501..2605                       /gene="bla"                       /note="AmpR promoter"       CDS             2606..3466                       /codon_start=1                       /gene="bla"                       /product="beta-lactamase"                       /note="AmpR"                       /note="confers resistance to ampicillin, carbenicillin, and                       related antibiotics"                       /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                       ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS                       PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                       EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                       LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                       LIKHW"       rep_origin      3637..4225                       /direction=RIGHT                       /note="ori"                       /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                       replication"  ORIGIN          1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag         61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt        121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac        181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg        241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag        301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat        361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat        421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt        481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga        541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg        601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg        661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc        721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg        781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt        841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt        901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg        961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa       1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc       1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc       1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagaggg       1201 cccgtttctg ctagcaagct tgctagcggc cgctcgaggc cggcaaggcc ggatcccccg       1261 acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt       1321 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga gatccctcgg       1381 agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct       1441 ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca       1501 actgaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa       1561 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt       1621 aaaagggtcc taaggaacag cgatgtaggt gggcgggcca agataggggc gcgattgctg       1681 cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc       1741 ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac       1801 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc       1861 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc       1921 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc       1981 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc       2041 ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc       2101 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa       2161 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa       2221 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa       2281 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa       2341 tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga gaattaattc       2401 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat       2461 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt       2521 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct       2581 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc       2641 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa       2701 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg       2761 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt       2821 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg       2881 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac       2941 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc       3001 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa       3061 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc       3121 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt       3181 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga       3241 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa       3301 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa       3361 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa       3421 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt       3481 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt       3541 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg       3601 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt       3661 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca       3721 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac       3781 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac       3841 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct       3901 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg       3961 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca       4021 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt       4081 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta       4141 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc       4201 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc       4261 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa       4321 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag       4381 cgagtcagtg agcgaggaag c  //

Key Applications

  • High-throughput recombinant protein screening.
  • Rapid production of monoclonal antibodies (mAbs).
  • Large-scale transient transfection in HEK293-6E systems.
Storage & Handling: Store at -20°C. We recommend using Endo-free plasmid kits for optimal transfection results.
Safety: For laboratory research use only. Not for human or animal diagnostic/therapeutic use.
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Technical Principle & Methodology

The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway: Recombinant DNA Molecular Cloning and Expression Vector Architecture (重组DNA分子克隆与表达载体工程构建技术). This ensures maximum reproducibility across clinical research and scientific workflows.

Standardized Experimental Assay Parameter
Vector Functionality Mammalian / Bacterial / Lentiviral Expression Vector
Promoter System CMV / T7 / T3 / SP6 Transcriptional Control
Selection Antibiotic Ampicillin / Kanamycin / Puromycin Stable Selection
Replicon Copy Number High-copy plasmid architecture for industrial yields
Format 2ug
Storage -20 Celsius degree
Resistance Ampicillin
Host HEK293, CHO, HEK293-6E
Promoter CMV
Replicon pMB1, oriP