Rabbit 6- phosphofructokinase, muscle type, PFKM ELISA KIT

Product Name:Rabbit 6- phosphofructokinase, muscle type, PFKM ELISA KIT
Packing:96T

Catalog No.:ELI-02495Ra

Gene Name:PFKM

Detect Range:31.2-2000 pg/mL

Sensitivity:20 pg/mL

Target Protein Name:Rabbit 6- phosphofructokinase, muscle type

Alternative Name:PFKM,Rabbit 6- phosphofructokinase, muscle type
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Rabbit 6- phosphofructokinase, muscle type, PFKM ELISA KIT allows for the in vitro quantitative determination ofRabbit 6- phosphofructokinase, muscle type concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Rabbit 6- phosphofructokinase, muscle type, PFKM ELISA KIT has been pre-coated with an PFKM antibody specific to Rabbit 6- phosphofructokinase, muscle type .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rabbit 6- phosphofructokinase, muscle type and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rabbit 6- phosphofructokinase, muscle type, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rabbit 6- phosphofructokinase, muscle type in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!