Rabbit Cystatin-C, Cys-C ELISA Kit
FOR RESEARCH USE ONLY!
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Rabbit Cys-C ELISA KIT allows for the in vitro quantitative determination of rabbit Cystatin-C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
The microtiter plate provided in Rabbit Cys-C ELISA KIT has been pre-coated with an antibody specific to Cystatin-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cystatin-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cystatin-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cystatin-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rabbit Cys-C ELISA KIT manual download
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