Rabbit Cystatin-C, Cys-C ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Rabbit Cys-C ELISA KIT ,Rabbit CysC ELISA KIT ,Rabbit Cystatin C ELISA KIT ,Rabbit ARMD11 ELISA KIT ,Rabbit HEL-S-2 ELISA KIT ,Rabbit cystatin 3 ELISA KIT ,Rabbit cystatin-3 ELISA KIT ,Rabbit epididymis secretory protein Li 2 ELISA KIT ,Rabbit gamma-trace ELISA KIT ,Rabbit neuroendocrine basic polypeptide ELISA KIT ,Rabbit post-gamma-globulin ELISA KIT ,Rabbit  Cystatin-C ELISA KIT ,Rabbit Cystatin-3 ELISA KIT ,Rabbit Gamma-trace ELISA KIT

Intended use

Rabbit Cys-C ELISA KIT allows for the in vitro quantitative determination of rabbit Cystatin-C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Rabbit Cys-C ELISA KIT has been pre-coated with an antibody specific to Cystatin-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cystatin-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cystatin-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cystatin-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.15-10ng/ml

Rabbit Cys-C ELISA KIT manual download

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