Rabbit p53/ TP53 ELISA Kit

Product Name:Rabbit p53/ TP53 ELISA Kit
Packing:96T

Catalog No.:Rabbit p53/ TP53 ELISA Kit

Gene Name:Rabbit p53/TP53

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Rabbit p53/TP53Rabbit p53/TP53
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Rabbit p53/ TP53 ELISA KitRabbit p53/TP53 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Rabbit p53/ TP53 ELISA Kit has been pre-coated with an Rabbit p53/TP53 antibody specific to Rabbit p53/TP53 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rabbit p53/TP53 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rabbit p53/TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rabbit p53/TP53 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!