Rabbit Soluble protein-100B, S-100B ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Protein S100-B,S100 calcium-binding protein B,S-100 protein beta chain,S-100 protein subunit beta, S100B,protein S100-B; NEF; S100;
S100-B; S100beta; S-100 calcium-binding protein, beta chain; S-100 protein subunit beta; S100 calcium-binding protein, beta (neural);
S100 calcium binding protein B

Search name
Rabbit Protein S100-B ELISA Kit, Rabbit Soluble protein-100B ELISA Kit, Rabbit S-100B ELISA Kit, Rabbit S100
calcium-binding protein B ELISA Kit, Rabbit S-100 protein beta chain ELISA Kit, Rabbit S-100 protein subunit beta ELISA Kit,
Rabbit S100B ELISA Kit, Rabbit protein S100-B ELISA Kit, Rabbit NEF ELISA Kit, Rabbit S100 ELISA Kit, Rabbit S100-B ELISA
Kit, Rabbit S100beta ELISA Kit, Rabbit S-100 calcium-binding protein beta chain ELISA Kit, Rabbit S-100 protein subunit beta
ELISA Kit, Rabbit S100 calcium-binding protein, beta (neural) ELISA Kit, Rabbit S100 calcium binding protein B ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rabbit Protein S100-B concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein S100-B. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein S100-B and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Protein S100-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein S100-B in the samples is then
determined by comparing the O.D. of the samples to the standard curve.