Rabbit tissue inhibitors of metalloproteinase, TIMP1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TIMP1,TIMP-1,CLGI; EPA; EPO; HCI; TIMP; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; metalloproteinase inhibitor 1; tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1

Search name

Rabbit TIMP1 ELISA KIT ,Rabbit TIMP-1 ELISA KIT ,Rabbit CLGI ELISA KIT ,Rabbit EPA ELISA KIT ,Rabbit EPO ELISA KIT ,Rabbit HCI ELISA KIT ,Rabbit TIMP ELISA KIT ,Rabbit collagenase inhibitor ELISA KIT ,Rabbit erythroid potentiating activity ELISA KIT ,Rabbit erythroid-potentiating activity ELISA KIT ,Rabbit fibroblast collagenase inhibitor ELISA KIT ,Rabbit metalloproteinase inhibitor 1 ELISA KIT ,Rabbit tissue inhibitor of metalloproteinases 1 ELISA KIT ,Rabbit TIMP metallopeptidase inhibitor 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rabbit TIMP-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metalloproteinase inhibitor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Metalloproteinase inhibitor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Metalloproteinase inhibitor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Metalloproteinase inhibitor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.