Rabbit TNF- Beta ELISA Kit

Product Name:Rabbit TNF- Beta ELISA Kit
Packing:96T

Catalog No.:Rabbit TNF- Beta ELISA Kit

Gene Name:Rabbit TNF-Beta

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Rabbit TNF-BetaRabbit TNF-Beta
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Rabbit TNF- Beta ELISA KitRabbit TNF-Beta concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Rabbit TNF- Beta ELISA Kit has been pre-coated with an Rabbit TNF-Beta antibody specific to Rabbit TNF-Beta .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rabbit TNF-Beta and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rabbit TNF-Beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rabbit TNF-Beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!