Rat Aldo- keto reductase family 1 member C21, Akr1c21 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Dihydrodiol dehydrogenase type 3,DD3,Dihydrodiol dehydrogenase type 3,DD3,Dihydrodiol dehydrogenase type 1,DD1,17-alpha-hydroxysteroid dehydrogenase,17-alpha-HSD,1.1.1.209,3-alpha-hydroxysteroid dehydrogenase,
9430025F20Rik,AI315367,17-alpha-hydroxysteroid dehydrogenase,3 alpha-hydroxysteroid dehydrogenase,17-alpha-hydroxysteroid dehydrogenase,3-alpha-hydroxysteroid dehydrogenase,DD1,DD3,aldo-keto reductase family 1 member C21,dihydrodiol dehydrogenase type 1,dihydrodiol dehydrogenase type 3
Search name
Rat Dihydrodiol dehydrogenase type 3 ELISA KIT,Rat DD3 ELISA KIT,Rat Dihydrodiol dehydrogenase type 3 ELISA KIT,Rat DD3 ELISA KIT,Rat Dihydrodiol dehydrogenase type 1 ELISA KIT,Rat DD1 ELISA KIT,Rat 17-alpha-hydroxysteroid dehydrogenase ELISA KIT,Rat 17-alpha-HSD ELISA KIT,Rat 1.1.1.209 ELISA KIT,Rat 3-alpha-hydroxysteroid dehydrogenase ELISA KIT,Rat 9430025F20Rik ELISA KIT,Rat AI315367 ELISA KIT,Rat 17-alpha-hydroxysteroid dehydrogenase ELISA KIT,Rat 3 alpha-hydroxysteroid dehydrogenase ELISA KIT,Rat 17-alpha-hydroxysteroid dehydrogenase ELISA KIT,Rat 3-alpha-hydroxysteroid dehydrogenase ELISA KIT,Rat DD1 ELISA KIT,Rat DD3 ELISA KIT,Rat aldo-keto reductase family 1 member C21 ELISA KIT,Rat dihydrodiol dehydrogenase type 1 ELISA KIT,Rat dihydrodiol dehydrogenase type 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Aldo- keto reductase family 1 member C21,AKR1C21 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AKR1C21, During the reaction, AKR1C21 in the sample or standard competes with a fixed amount of biotin-labeled AKR1C21 for sites on a pre-coated Monoclonal antibody specific to AKR1C21. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AKR1C21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Delivery time 4-6month