Rat Arachidonate 12- lipoxygenase, 12S- type, ALOX12 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY ELISA KIT, NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ALOX12 ELISA KIT,12-LOX ELISA KIT, 12S-LOX ELISA KIT, LOG12 ELISA KIT, 12(S)-lipoxygenase ELISA KIT, 12S-lipoxygenase ELISA KIT, arachidonate 12-lipoxygenase ELISA KIT, 12S-type ELISA KIT, platelet 12-LOX ELISA KIT, platelet-type 12-lipoxygenase ELISA KIT, platelet-type lipoxygenase 12 ELISA KIT, arachidonate 12-lipoxygenase ELISA KIT, 12-lipoxygenase ELISA KIT,LOX-12
Search name
Rat ALOX12 ELISA KIT ,Rat 12-LOX ELISA KIT ,Rat 12S-LOX ELISA KIT ,Rat LOG12 ELISA KIT ,Rat 12(S)-lipoxygenase ELISA KIT ,Rat 12S-lipoxygenase ELISA KIT ,Rat arachidonate 12-lipoxygenase ELISA KIT ,Rat 12S-type ELISA KIT ,Rat platelet 12-LOX ELISA KIT ,Rat platelet-type 12-lipoxygenase ELISA KIT ,Rat platelet-type lipoxygenase 12 ELISA KIT ,Rat arachidonate 12-lipoxygenase ELISA KIT ,Rat 12-lipoxygenase ELISA KIT ,Rat LOX-12 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat arachidonate 12-lipoxygenase ELISA KIT, 12s-type ELISA KIT,12S-lipoxygenase concentrations in serum ELISA KIT, Plasma ELISA KIT, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to 12S-lipoxygenase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 12S-lipoxygenase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 12S-lipoxygenase ELISA KIT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 12S-lipoxygenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.