Rat Alpha- actinin- 4, ACTN4 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Alpha-actinin-4,F-actin cross-linking protein,Non-muscle alpha-actinin 4, ACTN4,ACTININ-4; FSGS; FSGS1; F-actin cross-linking protein; actinin alpha4 isoform; alpha-actinin-4; focal segmental glomerulosclerosis 1; non-muscle alpha-actinin 4; actinin alpha 4

Search name

Rat Alpha-actinin-4 ELISA KIT ,Rat F-actin cross-linking protein ELISA KIT ,Rat Non-muscle alpha-actinin 4 ELISA KIT ,Rat ACTN4 ELISA KIT ,Rat ACTININ-4 ELISA KIT ,Rat FSGS ELISA KIT ,Rat FSGS1 ELISA KIT ,Rat F-actin cross-linking protein ELISA KIT ,Rat actinin alpha4 isoform ELISA KIT ,Rat alpha-actinin-4 ELISA KIT ,Rat focal segmental glomerulosclerosis 1 ELISA KIT ,Rat non-muscle alpha-actinin 4 ELISA KIT ,Rat actinin alpha 4 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat alpha-actinin-4,Alpha-actinin-4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-actinin-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-actinin-4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-actinin-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-actinin-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.