Rat Apolipoprotein A- II, APOA2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
APOA2, Apo-AII; ApoA-II; apoAII; apolipoprotein A2; apolipoprotein A-II,
Search name
Rat APOA2 ELISA KIT ,Rat Apo-AII ELISA KIT ,Rat ApoA-II ELISA KIT ,Rat apoAII ELISA KIT ,Rat apolipoprotein A2 ELISA KIT ,Rat apolipoprotein A-II ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Apolipoprotein A-II, Apo-AII concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo-AII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo-AII and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo-AII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apo-AII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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