Rat Complement C1q tumor necrosis factor- related protein 1, C1QTNF1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

C1QTNF1,CTRP1; GIP; ZSIG37; G protein coupled receptor interacting protein; complement C1q tumor necrosis factor-related protein 1; g protein-coupled receptor-interacting protein; C1q and tumor necrosis factor related protein 1

Search name

Rat C1QTNF1 ELISA KIT ,Rat CTRP1 ELISA KIT ,Rat GIP ELISA KIT ,Rat ZSIG37 ELISA KIT ,Rat G protein coupled receptor interacting protein ELISA KIT ,Rat complement C1q tumor necrosis factor-related protein 1 ELISA KIT ,Rat g protein-coupled receptor-interacting protein ELISA KIT ,Rat C1q and tumor necrosis factor related protein 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Complement C1q tumor necrosis factor-related protein 1, C1QTNF1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1QTNF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C1QTNF1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C1QTNF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C1QTNF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.