Rat Voltage-dependent N-type calcium channel subunit alpha-1B, CACNA1B ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Rat CACNA1B ELISA KIT ,Rat BIII ELISA KIT ,Rat CACNL1A5 ELISA KIT ,Rat CACNN ELISA KIT ,Rat Cav2.2 ELISA KIT ,Rat DYT23 ELISA KIT ,Rat Cav2.2 voltage-gated Ca2+ channel ELISA KIT ,Rat brain calcium channel III ELISA KIT ,Rat calcium channel alpha12.2 subunit ELISA KIT ,Rat calcium channel L type alpha-1 polypeptide ELISA KIT ,Rat calcium channel voltage-dependent L type alpha 1B subunit ELISA KIT ,Rat calcium channel voltage-dependent N type alpha 1B subunit ELISA KIT ,Rat voltage-gated calcium channel alpha subunit Cav2.2 ELISA KIT ,Rat voltage-gated calcium channel subunit alpha Cav2.2 ELISA KIT ,Rat calcium voltage-gated channel subunit alpha1 B ELISA KIT

Intended use

Rat CACNA1B ELISA kit allows for the in vitro quantitative determination of Rat Voltage-dependent N-type calcium channel subunit alpha-1B concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Rat CACNA1B ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CACNA1B, During the reaction, CACNA1B in the sample or standard competes with a fixed amount of biotin-labeled CACNA1B for sites on a pre-coated Monoclonal antibody specific to CACNA1B. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CACNA1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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Delivery time 4-6month

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