Rat Caveolin-1, CAV ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Caveolin-1,CAV1,caveolin-1,BSCL3; CGL3; MSTP085; PPH3; VIP21; cell growth-inhibiting protein 32; caveolin 1, caveolae protein, 22kDa

Search name

Rat CAV1 ELISA KIT ,Rat CAV ELISA KIT,Rat caveolin-1 ELISA KIT ,Rat BSCL3 ELISA KIT ,Rat CGL3 ELISA KIT ,Rat MSTP085 ELISA KIT ,Rat PPH3 ELISA KIT ,Rat VIP21 ELISA KIT ,Rat cell growth-inhibiting protein 32 ELISA KIT ,Rat caveolin 1 ELISA KIT ,Rat caveolae protein ELISA KIT  

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Caveolin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caveolin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Caveolin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Caveolin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Caveolin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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