Rat Cholecalciferol, toxiferol ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Cholecalciferol, toxiferol, VD3 ,vitamin D3, activated 7-dehydrocholesterol

Search name

Rat Cholecalciferol ELISA KIT ,Rat toxiferol ELISA KIT ,Rat VD3 ELISA KIT ,Rat vitamin D3 ELISA KIT ,Rat activated 7-dehydrocholesterol ELISA KIT

Intended use

Rat Cholecalciferol ELISA KIT allows for the in vitro quantitative determination of Rat Cholecalciferol concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay .The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D3 During the reaction, Vitamin D3 in the sample or standard competes with a fixed amount of biotin-labeled Vitamin D3 for sites on a pre-coated Monoclonal antibody specific to Vitamin D3. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Vitamin D3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 1.56-100 ng/ml

PDF Manual download

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