Rat Cluster of differentiation 30 ligand, CD30L ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Tumor necrosis factor ligand superfamily member 8,CD30 ligand(CD30-L), CD30 ligand,CD30L; CD153; CD30LG; CD153 antigen; CD30
antigen ligand; CD30-L; tumor necrosis factor ligand superfamily member 8; tumor necrosis factor (ligand) superfamily, member 8

Search name
Rat Tumor necrosis factor ligand superfamily member 8 ELISA Kit, Rat TNFL8 ELISA Kit, Rat CD30 ligand ELISA Kit,
Rat Cluster of differentiation 30 ligand ELISA Kit, Rat CD30L ELISA Kit, Rat CD30-L ELISA Kit, Rat CD153 ELISA
Kit, Rat CD30LG ELISA Kit, Rat CD153 antigen ELISA Kit, Rat D30 antigen ligand ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat tumor necrosis factor ligand superfamily member
8,TNFL8 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFL8. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TNFL8 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain TNFL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TNFL8 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.