Rat Cold- inducible RNA- binding protein, CIRBP ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Cold-inducible RNA-binding protein,A18 hnRNP,Glycine-rich RNA-binding protein CIRP, Cirbp,cold inducible RNA binding protein,CIRP; A18 hnRNP; cold inducible RNA-binding protein; cold-inducible RNA-binding protein; glycine-rich RNA binding protein; glycine-rich RNA-binding protein CIRP,

Search name

Rat Cold-inducible RNA-binding protein ELISA KIT ,Rat A18 hnRNP ELISA KIT ,Rat Glycine-rich RNA-binding protein CIRP ELISA KIT ,Rat  Cirbp ELISA KIT ,Rat cold inducible RNA binding protein ELISA KIT ,Rat CIRP ELISA KIT ,Rat A18 hnRNP ELISA KIT ,Rat cold inducible RNA-binding protein ELISA KIT ,Rat cold-inducible RNA-binding protein ELISA KIT ,Rat glycine-rich RNA binding protein ELISA KIT ,Rat glycine-rich RNA-binding protein CIRP ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat cold-inducible rna-binding protein,Cold-inducible RNA-binding protein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cold-inducible RNA-binding protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cold-inducible RNA-binding protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cold-inducible RNA-binding protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cold-inducible RNA-binding protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.