Rat Cyclin- dependent kinase 5 activator 1, CDK5R1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CDK5R1,CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a; CDK5 activator 1; TPKII regulatory subunit; cyclin-dependent kinase 5 activator 1; cyclin-dependent kinase 5 regulatory subunit 1; neuronal CDK5 activator; regulatory partner for CDK5 kinase; tau protein kinase II 23kDa subunit; cyclin-dependent kinase 5, regulatory subunit 1 (p35),Cyclin-dependent kinase 5 activator 1(CDK5 activator 1),Cyclin-dependent kinase 5 regulatory subunit 1,TPKII regulatory subunit,;

Synonyms

Rat CDK5R1 ELISA KIT ,Rat CDK5P35 ELISA KIT ,Rat CDK5R ELISA KIT ,Rat NCK5A ELISA KIT ,Rat p23 ELISA KIT ,Rat p25 ELISA KIT ,Rat p35 ELISA KIT ,Rat p35nck5a ELISA KIT ,Rat CDK5 activator 1 ELISA KIT ,Rat TPKII regulatory subunit ELISA KIT ,Rat cyclin-dependent kinase 5 activator 1 ELISA KIT ,Rat cyclin-dependent kinase 5 regulatory subunit 1 ELISA KIT ,Rat neuronal CDK5 activator ELISA KIT ,Rat regulatory partner for CDK5 kinase ELISA KIT ,Rat tau protein kinase II 23kDa subunit ELISA KIT ,Rat cyclin-dependent kinase 5 regulatory subunit 1 (p35) ELISA KIT ,Rat Cyclin-dependent kinase 5 activator 1 ELISA KIT ,Rat CDK5 activator 1 ELISA KIT ,Rat Cyclin-dependent kinase 5 regulatory subunit 1 ELISA KIT ,Rat TPKII regulatory subunit ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Cyclin-dependent kinase 5 activator 1, CDK5 activator 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDK5 activator 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CDK5 activator 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CDK5 activator 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CDK5 activator 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF Manual download