Rat Cystatin B ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat CSTB ELISA KIT ,Rat CPI-B ELISA KIT ,Rat CST6 ELISA KIT ,Rat EPM1 ELISA KIT ,Rat EPM1A ELISA KIT ,Rat PME ELISA KIT ,Rat STFB ELISA KIT ,Rat ULD ELISA KIT ,Rat cystatin-B ELISA KIT ,Rat cystatin B (stefin B) ELISA KIT ,Rat liver thiol proteinase inhibitor ELISA KIT ,Rat cystatin B ELISA KIT
Intended use
Rat Cystatin B ELISA kit allows for the in vitro quantitative determination of Rat Cystatin B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Rat Cystatin B ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cystatin B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cystatin B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cystatin B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat Cystatin B ELISA kit Range 78-5000pg/ml
Order Cystatin B ELISA kit by [email protected]
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