96 Tests

Alternative name

Hepcidin,
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Intended use

Rat Cyp3a9 ELISA kit allows for the in vitro quantitative determination of Rat Cyp3a9 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Rat Cyp3a9 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cyp3a9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyp3a9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cyp3a9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat Cyp3a9 ELISA kit  Range 78-5000pg/ml

Rat Cyp3a9 ELISA kit  Manual

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